Micropyramids to capture living cells

While pyramids are generally considered to be a grandeur construction, they are being used on micro level to capture live cells. The underlying principle is that since these micro pyramids have open walls, it is easier to living cells to interact-and easier for us to study them. This has been made possible through 3D Nano Fabrication.

Scientists of the research institutes MESA+ and MIRA of the University of Twente in The Netherlands present this new technology and first applications in the journal Small.

Most cell studies take place in 2-D: this is not a natural situation, because cells organize themselves in differently in the human body. If you give the cells room to move in three dimensions, the set-up is closer to what we find in nature. This is possible in the ‘open pyramids’ fabricated in the NanoLab of the MESA+ Institute for Nanotechnology of the University of Twente.

How is the pyramid being used?

If you join a number of flat silicon surfaces to form a sharp corner, it is possible to deposit another material on them. After having removed the the bulk of the material, however, a small amount of material remains in the corner. This tiny tip can be used for an Atomic Force Microscope, or, in this case, for forming a micro pyramid.

How are the cells captured?

In cooperation with UT’s MIRA Institute for Biomedical Technology and Technical Medicine, the nanoscientists have explored the possibilities of applying the pyramids as ‘cages’ for cells. First experiments with polystyrene balls worked out well. The next experiments involved capturing chondrocytes, cells forming cartilage. Moved by capillary fluid flow, these cells automatically ‘fall’ into the pyramid through a hole at the bottom. Soon after they settle in their 3-D cage, cells begin to interact with cells in adjacent pyramids. Changes in the phenotype of the cell can now be studied in a better way than in the usual 2-D situation. It is therefore a promising tool to be used in tissue regeneration research.

Possible Extension of the Idea

The Dutch scientists expect to develop extensions to this technology: the edges of the pyramid can be made hollow and function as fluid channels. Between the pyramids, it is also possible to create nanofluidic channels, which could be used to feed the cells.

Article: Erwin J. W. Berenschot, Narges Burouni, Bart Schurink, Joost W. van Honschoten, Remco G. P. Sanders, Roman Truckenmuller, Henri V. Jansen, Miko C. Elwenspoek, Aart A. van Apeldoorn, Niels R. Tas. 3D Nanofabrication of Fluidic Components by Corner LithographySmall, 2012


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